False positive reactions rarely occur with monoclonal blood grouping reagents due to their low protein content. However, if a reagent control is required, e.g. when typing red cells from patients suspected of having auto-antibodies or serum protein abnormalities, Lorne Negative Control for Monoclonal Anti-D reagents is advised.

A positive result obtained with Lorne Negative Control in addition to those obtained with Lorne Monoclonal IgM Anti-D reagents indicates that the specimen is most likely reacting with components of the reagent diluent other than the antibodies. A negative reaction with this control offers assurance that the positive results obtained with Anti-D reagent are due to specific antigen-antibody interactions

Lorne Negative Control is for the control of Monoclonal Anti-D reagents and is formulated with the same concentrations of phosphate buffer, sodium chloride, bovine albumin and macromolecular potentiators as Lorne Monoclonal Anti-D reagents with just the antibodies omitted. The reagent is supplied at optimal dilution for use with all the recommended techniques stated below without the need for further dilution or addition.